cryomesh  (Precision X-Ray)

Journal: bioRxiv

Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy

doi: 10.64898/2026.04.25.720819

Figure Lengend Snippet: ( A ) Human pancreatic islets were isolated from donor pancreases by enzymatic distension of the head and tail, digestion in a Ricordi chamber with agitation, and density-gradient purification . ( B ) Human embryonic stem cells were expanded and differentiated into stem cell–derived β cell islets (SC-islets) using a six-stage growth factor protocol . ( C ) Vitrification on gold-coated copper (Cu-Au) CryoMesh involved stepwise CPA loading, transfer of islets or SC-islets onto the mesh, removal of excess CPA by wicking, rapid plunging into liquid nitrogen, long-term cryobanking, and stepwise CPA unloading after rewarming. ( D ) Potential clinical benefits of islet cryopreservation include increased donor organ utilization, cryobanking of large islet or SC-islets batches as single-patient doses, improved donor–recipient matching, pooled donor transplantation, flexible scheduling of procedures, more time for batch quality assessment and safety testing, opportunities for immune tolerance induction, and global distribution. CPA, cryoprotective agent; hESC, human embryonic stem cell; LN 2 , liquid nitrogen

Article Snippet: Non-autoclavable materials, such as 3D-printed vitrification-rewarming containers and the fabricated CryoMesh, were sterilized with an X-Rad 320 (Precision X-Ray, Inc.) at an irradiation distance of 50 cm, operating at 320 kV and 12.5 mA, with a 2 mm aluminum filter to deliver a total dose of 5,000 cGray.

Techniques: Isolation, Purification, Derivative Assay, Transplantation Assay

Journal: bioRxiv

Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy

doi: 10.64898/2026.04.25.720819

Figure Lengend Snippet: ( A ) Schematic of the CryoMesh design. Wire diameter (D) is 50 ± 5 µm, and pore size (P) is 50 ± 5 µm. ( B ) Loading capacity of 2×2 cm, 5×4 cm, and 7×4 cm meshes. Green lines indicate theoretical monolayer capacity for 150 µm islets; multilayer capacity extends to 500 µm thickness. Purple points show SC-islet quantities tested during development; red (SC-islet) and blue (islet) points and boxplots indicate quantities tested on 7×4 cm meshes (inset photographs of mesh formats). ( C ) Magnified images of islets vitrified on Cu-Au CryoMesh (transparent, arrows, left) versus frozen (opaque, right). Mesh pore size = 50 µm. ( D ) Cooling and rewarming rates for mesh materials (n = 6–9 per group; Kruskal–Wallis with pairwise Wilcoxon tests). ( E ) Cooling and rewarming rates across mesh sizes (n = 9–14 per group; one-way ANOVA). ( F ) Post-rewarming viability of SC-islet on different mesh types (n = 3–6 per group; Kruskal–Wallis with pairwise Wilcoxon tests). ( G ) Representative viability (AO/PI) confocal images of rewarmed SC-islet (AO, live cells in cyan; PI, dead cells in red). Scale bar, 75 µm. Data shown as box-and-whisker plots or mean ± SD. Al, aluminum; AO, acridine orange; Cu + Au, copper-gold; IEQ, islet equivalents; ns, not significant; PI, propidium iodide; SC, stem cell; SS, stainless steel.

Article Snippet: Non-autoclavable materials, such as 3D-printed vitrification-rewarming containers and the fabricated CryoMesh, were sterilized with an X-Rad 320 (Precision X-Ray, Inc.) at an irradiation distance of 50 cm, operating at 320 kV and 12.5 mA, with a 2 mm aluminum filter to deliver a total dose of 5,000 cGray.

Techniques: Pore Size, Whisker Assay